Day 1 :
GALLY” International Biomedical Research Institute Inc., San Antonio, TX, USA
Keynote: Implication of the Multitarget Therapies in the Context of the Aged Associated Oxidative Stress Induced Cellular and Subcellular Hypoperfusion, and Mitochondrial DNA deletion During the Development and Maturation of Alzheimer Disease: past present future
Time : 10:00-11:00
Dr. Gjumrakch Aliev, MD, PhD, President “GALLY” International Biomedical Research Institute Inc., San Antonio, Texas, USA. He also hold appointment with the University of Atlanta, Georgia, USA as a Professor of Cardiovascular, Neuropathology, Gerontology, Health Science and Healthcare Administration, and as a Leading Scientist , Institute of Physiologically Active Compounds, Russian Academy of Sciences, Moscow Region, Chernogolovka, Russian Federation. He received his MD in 1982, from the Baku Medical University (former USSR) with cum laude. Then he accomplished his PhD in Cardiovascular Diseases from the prestigious Russian Academy of the Medical Sciences, Moscow, Russia in 1988 with cum laude. He received postdoctoral training with Professor G. Burnstock in the University College of the London. He is an outstanding teacher, scholar, and a renowned scientist in the area of cellular molecular physiology, and cardiovascular, and neurodegeneration-mediated pathologies including Alzheimer disease (AD). He is nationally and internationally reputed in his area. He is one of most cited authors in his fields with high impact factors.
Background and Hypothesis: Oxidative stress induced cerebral hypoperfusion and mitochondrial failure appears to be a key pathogenic factor in the development of age-associated diseases, triggering mild cognitive impairment and eventual conversion to Alzheimer disease (AD). Mitochondrial integrity is associated with cellular viability.
Goal: We studied cellular and subcellular features of hippocampal neurons and microvessel mitochondrial lesions, oxidative stress markers and protein immunoreactivity in animal models that mimic MCI and/or AD. In addition, we studied the effects of dietary antioxidant treatment on neuronal mitochondrial ultrastructure in rats. The goals of the proposed study are to determine the role of mitochondria failure and neuronal damage during the maturation of AD-like pathology in rats and transgenic mice overexpressing either amyloid β precursor protein (AßPP).
Methods: In this project we applied the following methods: transmission electron microscopy (TEM) qualitative analysis; EM pre-embedding immunogold cytochemistry using probes for human wild type, 5kb deleted and mouse mtDNA and antibodies against cytochrome c oxidase. Quantitative morphometric analysis of the degree of mitochondrial lesions.
Summary of Results: there was a significantly higher degree of mitochondrial damage and mitochondrial DNA overproliferation and deletion in neurons and cerebrovascular wall cells in transgenic mice and aged untreated rats in comparison to age-matched controls and non-treated subjects. Mitochondrial abnormalities are associated with atherosclerotic lesions of brain microvessels.
Conclusion: based on this results our conclusion is that changes in mitochondrial morphology and mitochondrial DNA coexist with metabolic dysfunction in AD mice and age-associated neurodegeneration and may serve as diagnostic markers and treatment targets
University of Belgrade, Serbia
Keynote: Epileptic phenomena, differential diagnosis, delirium in case of an acute stroke patients that have been diagnosed with vascular dementia in the past
Time : 11:20-12:05
I was born in 1964, received my medical degree from the Faculty of Medicine at the University of Belgrade in 1989. In 1994, I became a neurologist. Over the course of my career, I took part in many additional courses, most notably: - a 6 month course in electroencephalography at the Institute of Neurology(1991) - a pediatric and neonatal EEG course at the University Hospital for pediatrics (1996) - ultrasonography of main head and neck blood vessels and transcranial doppler training (2000) In regards to my work experience, I have worked from 1989 until 2002 at the General Hospital in Pozarevac. In 2002, I moved to the Hospital for cerebrovascular diseases “Sveti Sava” in Belgrade, and have been there since. Today, my work mainly revolves around the latest treatments of blood vessel thrombosis, thrombolysis and thrombectomy. Since 2008, I have published numerous research papers both domestically and internationally. In 2014 I obtained the title of a primarius, the highest domestic distinction for a clinical physician.
Patients who have psychiatric illness, dementia or brain tumors can go through real diagnostic procedures and therapy for a long time. Non-convulsive epileptic seizures are often unrecogniozied as transient confusional conditions in younger patients,which requires the introduction of electrocenography into the protocol in our patients.
The main aim of this research is to monitor patients with dementia, with MMSE score from 16 to 22, when the patients’ age is between 68 and 75 years. All of the patients had vascular dementia earlier and were women. We conducted protocol diagnostics and gave psychiatric therapy that they otherwise received before arriving in our institution and consult. The protocol includes CT endocranium, Doppler MKSC, TCD. In general, patients have fallen into delirious conditions that had been more or less successfully treated with haloperidol, risperidone and benzodiazepines. This has been standard for years in the world, and we have included the mandatory EEG procedure for these patients. There were 25 patients who were monitored, and indicated a clear epileptic activity mainly of FT or TO regions. We started with a small dose of lamotrigine, and a gradual introduction of it. First of all, in the context of delirious conditions, these are visible hallucinations and lamentations, crying and sometimes fear. After the introduction of antiepileptics, such symptoms have disappeared, gradually, with patient dosing and neurophysiological monitoring.
1. Refractory Epilepsy in Adults after CVI-published in the E Cronic 2017
2.Refractory status epileptics in adults after CVI, best poster award roma 2016.international conference on epilepsy and treatment
3. Ischemic stroke and migraine journal of neurological sciences 2013.
4. Stroke and leukoencephalopathy journal of neurological sciences 2013
5. Headche,migraine,brain lesion and MRI study journal of headache and pain 2013
Area of research interest : Cerebrovascular duseases,epilepsy, Non Convulsive status epllepticus, electrocenography,tarnslent ischmic attacks
1University of Picardie Jules Verne, France
Time : 12:05-12:50
Olivier Balédent has completed his PhD in Biophysics and Radiology at Jules Verne University. He is currently working as an Assistant Professor at Amiens University Hospital in France. He is heading the Department of Medical Image Processing and Bio Flow Image research team. After Master’s degree in Informatics in Amiens, he completed a Postgraduate Diploma in the field of Image Processing in Lyon. The thesis subject was about CSF flow imaging using MRI technique. Now, at Amiens University Hospital, with clinicians, he continues to develop CSF research and applies non-invasive hydrodynamic approach in clinical practice. He is also a Biophysics Teacher at Medical School of University of Picardie Jules Verne.
The rapid amplitude change of the cerebral systolic arterial input flow increases the brain volume. Then cerebrospinal fluid (CSF) is quickly displaced out of the cranium toward the spinal canal; intracranial pressure (ICP) increase is therefore limited. Nevertheless, this first CSF response is also limited and has to be supplemented with the cerebral blood venous outflow. The venous contribution is slower than the CSF but at the end drains from the cranium all the blood input volume. Finally, due to the narrow aqueduct of Sylvius, a small CSF ventricular flows out of the fourth ventricle. Cerebral hydrodynamics knowledge has benefited considerably from the introduction of phase-contrast magnetic resonance imaging (PCMRI), the unique technique to investigate the small but rapid CSF oscillations. Using post-processing software, key parameters of flow can be easily calculated. In ten minutes CSF flow is quantified in the spinal subarachnoid spaces, the pontine cistern, the foramens of Magendie and the aqueduct of Sylvius. Blood flow is quantified in the internal carotid and the vertebral arteries, straight and sagittal sinus, jugular and epidural veins. These flows data can be functional information’s complementary to the morphological imaging to better investigate the cranio-spinal system in case of patients presented hydrocephalus, Chiari malformation, syringomyelia, cerebral hemorrhage, intracranial hyper or hypo tension. The objective of this presentation is to describe the power and the limit of such clinical 2D PCMRI protocol concerning CSF and blood flow investigations and present what we have found in different healthy and pathological populations.